c2c12 myotubes  (ATCC)

Journal: Molecules

Article Title: Anti-Atrophic Effects of Dichotomine B from Stellaria dichotoma During Starvation-Induced Skeletal Muscle Atrophy

doi: 10.3390/molecules30183839

Figure Lengend Snippet: The effects of S. dichotoma extract and its sub-fractions on the diameter of DEX-induced C2C12 myotubes. ( A ) Cell morphology and ( B ) relative changes in the diameter of DEX-induced C2C12 myotubes cotreated with each S. dichotoma extract (30 μg/mL) observed under a microscope (200×) and analyzed by ImageJ2 software. The data are presented as means ± SDs (n = 3). ## p < 0.01 vs. control. ** p < 0.01 vs. dexamethasone treatment. *** p < 0.001 vs. dexamethasone treatment.

Article Snippet: C2C12 myotube viability was measured by cell counting kit-8 (CCK-8) (Dojindo, CK04, Kumamoto, Japan).

Techniques: Microscopy, Software, Control

Journal: Molecules

Article Title: Anti-Atrophic Effects of Dichotomine B from Stellaria dichotoma During Starvation-Induced Skeletal Muscle Atrophy

doi: 10.3390/molecules30183839

Figure Lengend Snippet: The effects of isolated compounds 1 – 5 from S. dichotoma on the diameter of DEX-induced C2C12 myotubes. ( A ) The chemical structures of compounds 1 – 5 isolated from the n -butanol fraction. ( B ) The cell morphology and ( C ) relative changes in myotube diameter of DEX-induced C2C12 myotubes cotreated with each isolated compound ( 1 – 5 ) under a microscope (scale bar = 250 μm) and analyzed by ImageJ software. These results are presented as means ± SDs (n = 3). ## p < 0.01 vs. control. * p < 0.05 vs. dexamethasone treatment. ** p < 0.01 vs. dexamethasone treatment.

Article Snippet: C2C12 myotube viability was measured by cell counting kit-8 (CCK-8) (Dojindo, CK04, Kumamoto, Japan).

Techniques: Isolation, Microscopy, Software, Control

Journal: Molecules

Article Title: Anti-Atrophic Effects of Dichotomine B from Stellaria dichotoma During Starvation-Induced Skeletal Muscle Atrophy

doi: 10.3390/molecules30183839

Figure Lengend Snippet: The effects of dichotomine B during DEX-induced C2C12 myotube atrophy. ( A ) Immunofluorescence staining of MHC after cotreatment with DEX and dichotomine B (DB) (10 or 30 μM) in C2C12 myotubes for 24 h. ( B ) The MHC-positive area and ( C ) fusion index were analyzed and determined by ImageJ2 software. The effects of DB on muscle atrophy biomarkers in DEX-stimulated C2C12 myotubes. ( D ) Western blot analysis of MHC, Atrogin-1, and MuRF-1 from lysates of DEX-induced C2C12 myotubes cotreated with DB (10 or 30 μM) for 24 h. The experiment was performed in triplicate independently (from the left panel to the right panel). ( E ) The relative changes in the protein levels of MHC, Atrogin-1, and MuRF-1 in DB-treated myotubes. The experiments were performed in triplicate. The data are presented as means ± SDs (n = 3). # p < 0.05 vs. control. ## p < 0.01 vs. control. ### p < 0.001 vs. control. * p < 0.05 vs. dexamethasone treatment. ** p < 0.01 vs. dexamethasone treatment. *** p < 0.001 vs. dexamethasone treatment.

Article Snippet: C2C12 myotube viability was measured by cell counting kit-8 (CCK-8) (Dojindo, CK04, Kumamoto, Japan).

Techniques: Immunofluorescence, Staining, Software, Western Blot, Control

Journal: Molecules

Article Title: Anti-Atrophic Effects of Dichotomine B from Stellaria dichotoma During Starvation-Induced Skeletal Muscle Atrophy

doi: 10.3390/molecules30183839

Figure Lengend Snippet: The effects of dichotomine B on the myotube diameter and MHC expression in C2C12 myotubes under serum-free conditions. ( A ) Immunofluorescence staining of MHC after treatment with dichotomine B (DB) (1 or 10 μM) in C2C12 myotubes under serum-free conditions for 24 h. ( B ) The myotube diameter and ( C ) the fusion index were analyzed and determined by ImageJ2 software. ( D ) Western blot analysis of MHC, Atrogin-1, and MuRF-1 from lysates of C2C12 myotubes incubated with DB (1 or 10 μM) under starvation conditions for 24 h. The relative changes in the protein levels of ( E ) MHC, ( F ) FoxO3a, ( G ) Atrogin-1, and ( H ) MuRF-1 in DB-treated myotubes. The data are presented as means ± SDs (n = 3). # p < 0.05 vs. control. ### p < 0.001 vs. control. * p < 0.05 vs. starved myotubes. ** p < 0.01 vs. starved myotubes. *** p < 0.001 vs. starved myotubes.

Article Snippet: C2C12 myotube viability was measured by cell counting kit-8 (CCK-8) (Dojindo, CK04, Kumamoto, Japan).

Techniques: Expressing, Immunofluorescence, Staining, Software, Western Blot, Incubation, Control